4KKL

Structure of the E148A mutant of CLC-ec1 delta NC construct in 100mM fluoride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	231.420, 100.094, 170.226
Unit cell angles	90.00, 131.82, 90.00

Refinement procedure

Resolution	25.000 - 2.850
R-factor	0.2506
Rwork	0.248
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.850
Number of reflections	71781
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	22% PEG400, 50mM Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K