4KKC

Structure of the E148A mutant of CLC-ec1 deltaNC construct in 20mM Bromide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	77
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.919
Spacegroup name	C 1 2 1
Unit cell lengths	231.212, 99.236, 170.513
Unit cell angles	90.00, 132.00, 90.00

Refinement procedure

Resolution	29.529 - 3.180
R-factor	0.2327
Rwork	0.231
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.819
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.180
Number of reflections	93237
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	295	23% PEG400, 100mM Na/K tartrate, 50mM glycine , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K