4KK9

Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 2mM Bromide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.919
Spacegroup name	C 1 2 1
Unit cell lengths	231.617, 98.860, 170.394
Unit cell angles	90.00, 131.88, 90.00

Refinement procedure

Resolution	39.928 - 2.997
R-factor	0.2199
Rwork	0.217
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.301
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.000
Number of reflections	54728
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	295	24% PEG400, 100mM Na/K tartrate, 50mM Glycine, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K