4KK1
Crystal Structure of TSC1 core domain from S. pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.321, 254.829, 228.472 |
| Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
| Resolution | 39.820 - 3.300 |
| R-factor | 0.19042 |
| Rwork | 0.186 |
| R-free | 0.26769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.577 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.385 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 174204 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 60mM imidazole, 40mM CHES, 120mM NH4 Citrate, 400mM 3Na Citrate, 12% PEG MME 2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
