4KJQ

Structure of the CLC-ec1 deltaNC construct in 100mM fluoride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	C 1 2 1
Unit cell lengths	231.312, 100.150, 169.969
Unit cell angles	90.00, 132.02, 90.00

Refinement procedure

Resolution	39.365 - 2.875
R-factor	0.2218
Rwork	0.220
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.293
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.810
Number of reflections	70131
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	295	22% PEG400, 100mM Na/K tartrate, 100mM tris-SO4 , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K