4KJM
Crystal structure of the Staphylococcus aureus protein (NP_646141.1, domain 3912-4037) similar to streptococcal adhesins emb and ebhA/ebhB
Replaces: 1XVHExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-06 |
Detector | SBC-2 |
Wavelength(s) | 0.98011, 0.98031, 0.96487 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 50.783, 59.230, 88.186 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.280 - 2.000 |
R-factor | 0.18767 |
Rwork | 0.185 |
R-free | 0.22986 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.694 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.7.0029. HKL3000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.053 | 0.263 |
Number of reflections | 18652 | |
<I/σ(I)> | 55.027 | 12.7 |
Completeness [%] | 99.7 | 100 |
Redundancy | 18.3 | 16.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 277 | 2.5M NACL, 0.1M IMIDAZOLE, 0.2M ZN(ACO)2, PH 8.00, VAPOR DIFFUSION, TEMPERATURE 277K |