4KJM

Crystal structure of the Staphylococcus aureus protein (NP_646141.1, domain 3912-4037) similar to streptococcal adhesins emb and ebhA/ebhB

Replaces:  1XVH

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-06
Detector	SBC-2
Wavelength(s)	0.98011, 0.98031, 0.96487
Spacegroup name	P 21 21 2
Unit cell lengths	50.783, 59.230, 88.186
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.280 - 2.000
R-factor	0.18767
Rwork	0.185
R-free	0.22986
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.694
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	REFMAC (5.7.0029. HKL3000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.053	0.263
Number of reflections	18652
<I/σ(I)>	55.027	12.7
Completeness [%]	99.7	100
Redundancy	18.3	16.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	277	2.5M NACL, 0.1M IMIDAZOLE, 0.2M ZN(ACO)2, PH 8.00, VAPOR DIFFUSION, TEMPERATURE 277K