4KJI
Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 83.649, 83.649, 94.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 3.200 |
| R-factor | 0.226 |
| Rwork | 0.222 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1vpz for protein and 2JPP for RNA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.718 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.570 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.085 | |
| Number of reflections | 5917 | |
| <I/σ(I)> | 18.6 | |
| Redundancy | 8.8 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.5 | 0.08M Mg(OAc)2, 0.1M NaOAc pH 4.5, 4% benzamidine HCl, 4% PEG 8000., EVAPORATION |
