4KIV

RECOMBINANT KRINGLE IV-10/W72R MUTANT OF HUMAN APOLIPOPROTEIN(A)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	130
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	24.300, 45.820, 56.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.166 *
Rwork	0.162
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	0.032
Data reduction software	RIGAKU (R-AXIS)
Data scaling software	R-AXIS ((RIGAKU))
Phasing software	AMoRE
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.300	2.500
High resolution limit [Å]	2.200	2.200
Rmerge	0.089	0.089 *
Total number of observations	6494 *
Number of reflections	2241 *
<I/σ(I)>	7.2	2
Completeness [%]	64.0	45
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG8000	20 (%)
3	1	reservoir	sodium cacodylate	0.1 (M)
4	1	reservoir		0.1 (M)