4KIS
Crystal Structure of a LSR-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 290.750, 290.750, 290.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.860 - 3.200 |
| R-factor | 0.239 |
| Rwork | 0.236 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.118 | 0.791 |
| Number of reflections | 432015 | |
| <I/σ(I)> | 14.25 | |
| Completeness [%] | 96.8 | 66.2 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | HEPES, CaCl2, MPD, glycerol , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | HEPES, CaCl2, MPD, glycerol , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
