4KI9

Crystal structure of the catalytic domain of human DUSP12 at 2.0 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Spacegroup name	P 41 21 2
Unit cell lengths	50.550, 50.550, 128.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.698 - 2.000
R-factor	0.1738
Rwork	0.169
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.046
Data reduction software	MOSFLM
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.700
High resolution limit [Å]	2.000
Number of reflections	11473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			16% PEG 8000, 38% monopotassium phosphate, 20% glycerol, VAPOR DIFFUSION
1	VAPOR DIFFUSION			16% PEG 8000, 38% monopotassium phosphate, 20% glycerol, VAPOR DIFFUSION