4KG7
Structure of MycP3 protease from the type VII (ESX-3) secretion system.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.820, 46.950, 47.540 |
| Unit cell angles | 77.27, 68.35, 74.47 |
Refinement procedure
| Resolution | 43.820 - 1.500 |
| R-factor | 0.1527 |
| Rwork | 0.151 |
| R-free | 0.18460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hvl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 | |
| High resolution limit [Å] | 1.500 | 6.710 | 1.500 |
| Rmerge | 0.048 | 0.017 | 0.465 |
| Number of reflections | 48069 | 557 | 2252 |
| <I/σ(I)> | 15.56 | 47.62 | 2.04 |
| Completeness [%] | 91.4 | 94.4 | 57.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 295 | 0.2M MAGNESIUM CHLORIDE, 0.1M TRIS-HCL, 20% PEG8000, 0.01M NICKEL CHLORIDE, pH 8.4, vapor diffusion, hanging drop, temperature 295K |
