4KG6

Crystal Structure of AmpC beta-lactamase N152G Mutant from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-06-28
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	163.773, 79.660, 129.288
Unit cell angles	90.00, 104.01, 90.00

Refinement procedure

Resolution	27.500 - 1.750
R-factor	0.16147
Rwork	0.159
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.076
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	162537
Completeness [%]		99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	296	1.7M potassium phosphate, 3.5mg/ml AmpC, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 296K