4KG0
Crystal structure of the drosophila melanogaster neuralized-nhr1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.93, 0.95370 |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.280, 89.280, 36.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.660 - 2.100 |
| R-factor | 0.166 |
| Rwork | 0.163 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yue |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.455 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.372 |
| Number of reflections | 10026 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 10.5 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.4M MAGNESIUM NITRATE, 10% PEG 3350, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, PH 8.0 |
