4KFV
Structural insight into Golgi membrane stacking by GRASP65 and GRASP55
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Detector technology | CCD |
Collection date | 2012-09-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 44.989, 104.287, 37.932 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.063 - 2.200 |
R-factor | 0.2171 |
Rwork | 0.214 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.420 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Number of reflections | 9540 | ||
Completeness [%] | 99.3 | 98.2 | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 5.5 | 293 | 0.1 M Bis-Tris (pH 5.5) 25% PEG3350, LIQUID DIFFUSION, temperature 293K |