4KFS
Structure of the genome packaging NTPase B204 from Sulfolobus turreted icosahedral virus 2 in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.600, 61.015, 70.004 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 46.294 - 1.946 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kfr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.294 | 2.060 |
| High resolution limit [Å] | 1.946 | 1.946 |
| Rmerge | 0.083 | 0.680 |
| Number of reflections | 28041 | |
| <I/σ(I)> | 5.11 | 1.07 |
| Completeness [%] | 91.3 | 67.7 |
| Redundancy | 5.36 | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris-HCl, 0.2 M magnesium chloride, 30% PEG4000, 5 mM AMP, protein in 50 mM sodium citrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
