4KF7
Nup188(aa1-1160) from Myceliophthora thermophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.028, 94.773, 91.635 |
| Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
| Resolution | 66.759 - 2.650 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.22540 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.800 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.031 | 0.772 |
| Number of reflections | 41673 | |
| <I/σ(I)> | 31.3 | 1.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M MES, 4.5-7.0% w/v PEG4000, 150 mM ammonium sulfate, 1 mM DTT, 1.0-2.5% tert-butanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
