4KF5

Crystal Structure of Split GFP complexed with engineered sfCherry with an insertion of GFP fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Detector technology	CCD
Collection date	2011-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	74.360, 86.490, 167.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.995 - 2.599
R-factor	0.2055
Rwork	0.203
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.852
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: dev_1338))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.599	2.600
Rmerge	0.065	0.477
Number of reflections	31541
<I/σ(I)>	18.8	2.22
Completeness [%]	98.8	94
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	298	Bis-Tris Buffer pH 8.3, 20% PEG 3350, and 6% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K