4KEX

Crystal structure analysis of a single amino acid deletion mutation in EGFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Wavelength(s)	0.97630
Spacegroup name	P 21 21 21
Unit cell lengths	51.450, 63.140, 65.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.570 - 1.600
R-factor	0.1837
Rwork	0.182
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eul
RMSD bond length	0.023
RMSD bond angle	2.139
Data scaling software	SCALA (0.1.16)
Phasing software	PHASER (2.5.1)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	51.450
High resolution limit [Å]	1.600
Rmerge	0.092
Number of reflections	28209
<I/σ(I)>	15.2
Completeness [%]	98.1
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	291	0.1 M MMT Buffer (Malic acid, MES and Tris), 25% (w/v) PEG 1500, pH 4.0, VAPOR DIFFUSION, temperature 291K