Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.751, 39.198, 84.200 |
| Unit cell angles | 90.00, 117.68, 90.00 |
Refinement procedure
| Resolution | 36.144 - 1.830 |
| R-factor | 0.1763 |
| Rwork | 0.174 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aj3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.053 | 0.405 |
| Number of reflections | 21861 | |
| <I/σ(I)> | 19.7 | 5.65 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 30% PEG4000, 0.2M ammonium acetate, 0.1M sodium acetate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
