4KED

Crystal Structure of Cofilin Mutant (cof1-157p)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	150.023, 30.325, 104.268
Unit cell angles	90.00, 133.01, 90.00

Refinement procedure

Resolution	27.427 - 1.901
R-factor	0.1845
Rwork	0.181
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.972
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	50075
Completeness [%]	94.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	20% PEG4000, 150mM NaCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K