4KDU
Crystal structure of a glutathione transferase family member from Burkholderia graminis, target efi-507264, no gsh, ordered domains, space group P21, form(1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-17 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.603, 56.105, 67.216 |
Unit cell angles | 90.00, 107.78, 90.00 |
Refinement procedure
Resolution | 24.196 - 1.600 |
R-factor | 0.1697 |
Rwork | 0.168 |
R-free | 0.20040 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.327 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 64.004 | 24.196 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.083 | 0.044 | 0.261 |
Total number of observations | 4829 | 22297 | |
Number of reflections | 57253 | ||
<I/σ(I)> | 7.3 | 14 | 2.9 |
Completeness [%] | 93.3 | 79.3 | 93.5 |
Redundancy | 2.8 | 3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | Protein (10 mM Hepes pH 7.5, 150 mM NaCl, 5% glycerol, 5 mM GSH); Reservoir (1 M di-Ammonium Phosphate 0.1 M Sodium Acetate); Cryoprotection (reservoir + 20% glycerol), VAPOR DIFFUSION, SITTING DROP, temperature 298K |