4KDS
Crystal structure of latent rainbow trout plasminogen activator inhibitor 1 (PAI-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2013-04-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.039 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.065, 56.471, 59.750 |
| Unit cell angles | 90.00, 93.88, 90.00 |
Refinement procedure
| Resolution | 20.219 - 1.668 |
| R-factor | 0.1748 |
| Rwork | 0.172 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.624 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.220 |
| High resolution limit [Å] | 1.650 |
| Number of reflections | 44752 |
| Completeness [%] | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10 % PEG3350, 0.2M K-thiocyanate, 0.1M Hepes 7.0 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
