4KDD
Structure of Mycobacterium tuberculosis ribosome recycling factor in presence of detergent
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.370, 33.720, 61.510 |
| Unit cell angles | 90.00, 112.84, 90.00 |
Refinement procedure
| Resolution | 33.700 - 1.900 |
| R-factor | 0.25052 |
| Rwork | 0.249 |
| R-free | 0.27048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wqg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.740 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.133 | 0.133 |
| Number of reflections | 17141 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil in microbatch plates | 7.8 | 298 | 6% polyethylene glycol 4000, 100mM Tris HCl, 0.6mM Cadmium acetate, 5% polyethylene glycol 400, pH 7.8, Under oil in microbatch plates, temperature 298K |
