4KC6
Crystal structure of C-terminal deletion mutant of ribosome recycling factor from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-31 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.990, 48.790, 84.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.210 - 2.400 |
| R-factor | 0.25357 |
| Rwork | 0.251 |
| R-free | 0.30398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wqg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.108 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.240 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.124 | 0.124 |
| Number of reflections | 7009 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil in microbatch plates | 7.8 | 298 | 6% Polyethylene glycol 4000, 0.1M Tris HCL, 0.6mM Cadmium acetate, 5% polyethylene glycol 400, pH 7.8, Under oil in microbatch plates, temperature 298K |
