4KBX

Crystal structure of the pyridoxal-5'-phosphate dependent protein yhfx from escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	66.920, 82.651, 149.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.460 - 1.599
R-factor	0.1734
Rwork	0.173
R-free	0.19790
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.073
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.460
High resolution limit [Å]	1.599
Number of reflections	53931
Completeness [%]	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.5M Lithium sulfate, 0.1M Hepes , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K