4KBP

KIDNEY BEAN PURPLE ACID PHOSPHATASE

Experimental procedure

Temperature [K]	277
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1995-04-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	132.700, 347.300, 128.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.192
Rwork	0.192
R-free	0.22700 *
RMSD bond length	0.020
RMSD bond angle	2.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.700
Rmerge	0.086
Total number of observations	329933 *
Number of reflections	71089
Completeness [%]	87.5	73.7 *
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	12 (mg/ml)
2	1	drop	PEG10000	4 (%)
3	1	drop	sodium acetate	50 (mM)
4	1	reservoir	PEG10000	15 (%)