4KBO

Crystal structure of the human Mortalin (GRP75) ATPase domain in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-14
Detector	NOIR-1
Spacegroup name	P 21 21 21
Unit cell lengths	51.197, 67.812, 120.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.380 - 2.800
R-factor	0.2214
Rwork	0.218
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e88
RMSD bond length	0.013
RMSD bond angle	0.860
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHENIX ((1.8.1_1168))
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.384	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	10669
Completeness [%]	98.0	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% PEG 3350, 0.2M di-ammonium tartrate, 0.02M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K