4KBJ

Structure of Mtb RNAP Beta subunit B1 and B2 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	53.183, 123.911, 135.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.513 - 2.453
R-factor	0.2161
Rwork	0.214
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.681
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.513	2.540
High resolution limit [Å]	2.450	2.450
Number of reflections	33628
<I/σ(I)>		1.9
Completeness [%]	99.5	97
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	289	20% PEG 3350, 0.2 M potassium citrate tribasic monohydrate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K