4K9E

Crystal structure of KIT D4D5 fragment in complex with anti-Kit antibodies Fab79D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-18
Detector	ADSC QUANTUM 315
Spacegroup name	P 65 2 2
Unit cell lengths	94.577, 94.577, 320.876
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.779 - 2.700
R-factor	0.2484
Rwork	0.247
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.756
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge		0.040	0.518
Number of reflections	24161
<I/σ(I)>	10.1
Completeness [%]	98.8	99.3	89.8
Redundancy	7.8	7.7	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2		20-24% PEG 400, 0.1 M Tris-HCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP