4K92

A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-10-01
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.980377
Spacegroup name	P 21 21 21
Unit cell lengths	62.284, 66.492, 138.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.997 - 2.005
R-factor	0.1846
Rwork	0.183
R-free	0.20990
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.151
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	36434
<I/σ(I)>		3.9
Completeness [%]	92.3	64.9
Redundancy	6.3	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	300	equal volume of 10 mg/ml hCLASP1 TOG2 and well solution containing 22% PEG3350, 200mM Sodium citrate, pH 8.25 were mixed together and equilibrated over 1 ml of well solution., VAPOR DIFFUSION, HANGING DROP, temperature 300K