4K8J
Crystal Structure of Staphylococcal nuclease mutant V23L/V66I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.200, 48.200, 63.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.550 - 2.000 |
| R-factor | 0.20331 |
| Rwork | 0.201 |
| R-free | 0.25108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.012 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.290 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.051 | 0.747 |
| Number of reflections | 15671 | |
| <I/σ(I)> | 0.4 | |
| Redundancy | 2.2 | 1.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 25mM Sodium Phosphate, 35-60% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
