4K83
Crystal structure of lv-ranaspumin (Lv-RSN-1) from the foam nest of Leptodactylus vastus, orthorhombic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9497 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.662, 52.900, 102.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.030 - 1.750 |
| R-factor | 0.2026 |
| Rwork | 0.200 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k82 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.359 | 47.030 | 1.840 |
| High resolution limit [Å] | 1.750 | 5.530 | 1.750 |
| Rmerge | 0.027 | 0.500 | |
| Number of reflections | 20733 | ||
| <I/σ(I)> | 43.6 | 14 | 1.6 |
| Completeness [%] | 99.5 | 99.9 | 98.7 |
| Redundancy | 25.4 | 21.2 | 26.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapour diffusion, hanging drop | 8 | 293 | 0.25 M sodium acetate, 28% PEG 3350, pH 8.0, vapour diffusion, hanging drop, temperature 293K |
