4K7Y
Oye1-w116t
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.88560 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 141.327, 141.327, 42.832 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.592 - 1.199 |
R-factor | 0.1174 |
Rwork | 0.115 |
R-free | 0.13520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.364 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.596 | 1.240 |
High resolution limit [Å] | 1.199 | 1.200 |
Rmerge | 0.067 | 0.570 |
Number of reflections | 135203 | |
<I/σ(I)> | 24.21 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 277 | 0.2M MgCl2, 0.1M HEPES, 30-40% PEG400, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |