4K7R
Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-03 |
| Detector | ACSD |
| Wavelength(s) | 0.98 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 89.026, 89.026, 474.103 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.711 - 2.094 |
| R-factor | 0.2157 |
| Rwork | 0.214 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.951 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.170 |
| High resolution limit [Å] | 2.090 | 4.350 | 2.090 |
| Rmerge | 0.087 | 0.040 | 0.404 |
| Number of reflections | 80183 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 97.3 | 96.2 | 97.2 |
| Redundancy | 2.4 | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 7% PEG3350, 0.2M NH4SO4, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, temperature 298K |
