4K7O
HUMAN PEROXIREDOXIN 5 with a fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.960, 101.550, 148.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.100 - 1.980 |
| Rwork | 0.176 |
| R-free | 0.21653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.100 | 65.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.100 | 0.100 |
| Number of reflections | 45081 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.3 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | 22% PEG3350, 0.1M sodium citrate buffer, 0.2M potassium sodium tartrate, 5mM 1,4-dithio-dl-threitol, 0.02%(w/v) sodium azide, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
