4K7M
Crystal structure of RNase S variant (K7C/Q11C) with bound mercury ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.870, 43.870, 96.361 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.930 - 1.800 |
| R-factor | 0.16684 |
| Rwork | 0.165 |
| R-free | 0.20456 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2RNS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.992 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.935 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 10484 | |
| Completeness [%] | 99.8 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 292 | 0.1 M Citric Acid, 2.1 M Ammonium Sulfate, pH 3.9, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
