4K7K

Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	62.014, 104.488, 72.063
Unit cell angles	90.00, 101.06, 90.00

Refinement procedure

Resolution	46.011 - 2.530
R-factor	0.2107
Rwork	0.208
R-free	0.26710
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.105
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.630
High resolution limit [Å]	2.530	5.260	2.530
Rmerge	0.069	0.027	0.417
Number of reflections	30128
<I/σ(I)>	8
Completeness [%]	94.0	97.3	91.6
Redundancy	2	2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	7% PEG3350, 0.2M NH4SO4, 0.1M HEPES (7.5), vapor diffusion, temperature 298K