4K7H
Major capsid protein P1 of the Pseudomonas phage phi6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97899 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 182.591, 278.852, 246.469 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.920 - 3.596 |
| R-factor | 0.2196 |
| Rwork | 0.217 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | cryoEM map |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.411 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 3.660 |
| High resolution limit [Å] | 3.596 | 3.596 |
| Rmerge | 0.192 | |
| Number of reflections | 66561 | |
| <I/σ(I)> | 11.15 | 2.04 |
| Completeness [%] | 91.1 | 41.67 |
| Redundancy | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 HEPES, pH 7.5, 180 mM calcium acetate, 10 mM EDTA, 39% PEG 400, 1:1 molar mixture with P7 protein, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
