4K7E

Crystal structure of Junin virus nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-18
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	I 21 21 21
Unit cell lengths	40.415, 78.472, 136.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.256 - 2.200
R-factor	0.1979
Rwork	0.197
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.004
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.260	2.240
High resolution limit [Å]	2.200	2.200
Rmerge		0.083
Number of reflections	11370
<I/σ(I)>		53.5
Completeness [%]	99.8	99.8
Redundancy		10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	0.1M Tris, 2.2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K