4K7C
Crystal structure of pepw from lactobacillus rhamnosis hn001 (dr20) determined as the selenomet derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 143.432, 143.432, 97.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.760 - 1.660 |
| R-factor | 0.17854 |
| Rwork | 0.177 |
| R-free | 0.20968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zuk |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.013 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.760 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.093 | 0.412 |
| Number of reflections | 84251 | |
| <I/σ(I)> | 5.9 | 1.2 |
| Completeness [%] | 87.4 | 23.1 |
| Redundancy | 3.22 | 1.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 0.1M NaAc, 11% Peg 4K, 30% glycerol protectant, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
