4K7B
Crystal structure of Extrinsic protein in photosystem II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.411, 92.411, 31.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.249 - 1.550 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.19490 |
| Structure solution method | SIRAS, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
| High resolution limit [Å] | 1.547 | 4.210 | 1.550 |
| Rmerge | 0.061 | 0.023 | 0.499 |
| Number of reflections | 22604 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.8 | 97.8 | 99.5 |
| Redundancy | 6.1 | 6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | pH 6.0, VAPOR DIFFUSION, SITTING DROP |
