4K70
Crystal Structure of N-terminal half of Pseudorabiesvirus UL37 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.670, 156.589, 67.381 |
| Unit cell angles | 90.00, 91.33, 90.00 |
Refinement procedure
| Resolution | 43.117 - 2.000 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.22030 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.939 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.975 | 1.975 |
| Rmerge | 0.085 | 0.513 |
| Number of reflections | 64382 | |
| <I/σ(I)> | 17.63 | 2 |
| Completeness [%] | 89.4 | 53.3 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 24% PEG 1000, 0.3M calcium acetate, 0.1M Imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
