4K6R
Crystal structure of GlmU in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 76.280, 76.280, 277.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.820 - 1.980 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dk5 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.157 |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 1.990 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 41778 | |
| Completeness [%] | 99.6 | 99.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 100mM Hepes (pH 7.5), 1mM DTT, 8% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
