4K64

Structure of an avian influenza H5 hemagglutinin from the influenza virus complexed with human receptor analog LSTc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-20
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 3
Unit cell lengths	70.336, 70.336, 491.793
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.042 - 2.604
R-factor	0.2241
Rwork	0.221
R-free	0.27990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fk0
RMSD bond length	0.003
RMSD bond angle	0.839
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	77142
Completeness [%]	92.6	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	25% PEG 2000 MME, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K