4K4S
Poliovirus polymerase elongation complex (r3_form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.768, 58.859, 97.586 |
| Unit cell angles | 77.88, 77.80, 79.97 |
Refinement procedure
| Resolution | 39.922 - 2.400 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.129 |
| Data reduction software | d*TREK (9.9.9.1L) |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.930 | 39.930 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.066 | 0.068 | 0.521 |
| Total number of observations | 9154 | 9087 | |
| Number of reflections | 47355 | ||
| <I/σ(I)> | 6.2 | 15.3 | 1.5 |
| Completeness [%] | 97.7 | 97 | 97.5 |
| Redundancy | 1.91 | 1.92 | 1.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | sitting drop, temperature 289K, VAPOR DIFFUSION, SITTING DROP |
