4K4S

Poliovirus polymerase elongation complex (r3_form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Detector	NOIR-1
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	58.768, 58.859, 97.586
Unit cell angles	77.88, 77.80, 79.97

Refinement procedure

Resolution	39.922 - 2.400
R-factor	0.2001
Rwork	0.198
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.129
Data reduction software	d*TREK (9.9.9.1L)
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.930	39.930	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.066	0.068	0.521
Total number of observations		9154	9087
Number of reflections	47355
<I/σ(I)>	6.2	15.3	1.5
Completeness [%]	97.7	97	97.5
Redundancy	1.91	1.92	1.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	sitting drop, temperature 289K, VAPOR DIFFUSION, SITTING DROP