4K46

Crystal Structure of Adenylate Kinase from Photobacterium profundum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979306
Spacegroup name	P 21 21 21
Unit cell lengths	42.590, 70.230, 71.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.160 - 2.010
R-factor	0.17
Rwork	0.167
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.208
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.160	2.120
High resolution limit [Å]	2.010	2.010
Rmerge	0.074
Number of reflections	14921
<I/σ(I)>	15.2	0.078
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	291	2.3M ammonium sulfate, 100mM imidazole, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K