4K3Y

Crystal structure of a subtype N11 neuraminidase-like protein of A/flat-faced bat/Peru/033/2010 (H18N11)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 61
Unit cell lengths	181.290, 181.290, 136.597
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.870 - 2.682
R-factor	0.1804
Rwork	0.178
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gdi
RMSD bond length	0.009
RMSD bond angle	1.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.730
High resolution limit [Å]	2.680	2.680
Number of reflections	70321
Completeness [%]	98.4	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	295	0.2 M potassium sulfate, 18% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 295K