4K3R
E. coli sliding clamp in complex with AcQLDLA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.960, 67.090, 81.180 |
| Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
| Resolution | 43.980 - 1.860 |
| R-factor | 0.2116 |
| Rwork | 0.209 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mmi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.068 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.265 | 43.985 | 1.960 |
| High resolution limit [Å] | 1.859 | 5.880 | 1.860 |
| Rmerge | 0.038 | 0.509 | |
| Rmeas | 0.045 | 0.604 | |
| Rpim | 0.024 | 0.323 | |
| Total number of observations | 7367 | 21862 | |
| Number of reflections | 61020 | ||
| <I/σ(I)> | 9.7 | 20.7 | 2.4 |
| Completeness [%] | 92.5 | 97.8 | 78.4 |
| Redundancy | 3.1 | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 100mM MES, 100-150mM CaCl2, 25-30%(v/v) PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
