4K3P

E. coli sliding clamp in complex with AcQLALF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-07-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	79.960, 66.680, 80.920
Unit cell angles	90.00, 114.16, 90.00

Refinement procedure

Resolution	49.490 - 2.150
R-factor	0.2256
Rwork	0.223
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mmi
RMSD bond length	0.006
RMSD bond angle	1.133
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.832	49.486	2.270
High resolution limit [Å]	2.150	6.800	2.150
Rmerge		0.033	0.411
Rmeas		0.041	0.501
Rpim		0.024	0.282
Total number of observations		4471	15049
Number of reflections	38882
<I/σ(I)>	9.7	23.2	2.2
Completeness [%]	91.7	98.8	88.5
Redundancy	3	3.2	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	285	100mM MES, 100-150mM CaCl2, 25-30%(v/v) PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K