4K3G
Immunoglobulin lambda variable domain L5(L89S) fluorogen activating protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 41 |
| Unit cell lengths | 54.860, 54.860, 95.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.430 - 1.930 |
| R-factor | 0.1948 |
| Rwork | 0.193 |
| R-free | 0.23120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.193 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.430 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.054 | 0.546 |
| Number of reflections | 21287 | |
| <I/σ(I)> | 23.3 | 3.1 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M sodium acetate, 10% 2-methyl-2,3-pentanediol , pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
